تغییرات آنزیم‌های مرتبط با دفاع در گیاهچه‌ چغندرقند رقم Brigita علیه ویروس‌ پیچیدگی شدید بوته چغندرقند (BSCTV) و باکتری Pseudomonas fluorescens استرین CHA0

نویسندگان

چکیده

تنش‌های مختلف محیطی شامل تنش‌های زنده و غیرزنده سبب تولید رادیکال‌های آزاد اکسیژن می‌شوند از جمله این تنش‌ها می‌توان به برهمکنش گیاه-ویروس اشاره کرد. گیاهان با دارا بودن سیستم ضد اکسنده که شامل ترکیبات آنزیمی و غیر آنزیمی می‌باشد معمولا سطوح رادیکال‌های آزاد اکسیژن را در سلول در حد متعادل نگه می‌دارند. هدف از انجام این تحقیق، بررسی اثر ویروس پیچیدگی شدید بوته چغندرقند (BSCTV) و همچنین اثر این ویروس به همراه باکتری Pseudomonas fluorescens استرین CHA0 بر نحوه فعالیت آنزیم‌های ضداکسنده در یک رقم حساس  چغندرقند (Brigita) می‌باشد فعالیت ترکیبات آنزیمی ضداکسنده در این بررسی با استفاده از دستگاه طیف سنج در دمای اتاق اندازه‌گیری شد. نتایج حاصل از بررسی نشان داد که وقتی علائم شدید آلودگی روی برگ‌ها گسترش می‌یاید و مقدار ویروس به حداکثر می‌رسد افزایش معنی‌داری در میزان فعالیت آنزیم‌های ضداکسنده آسکوربیت پراکسیداز، پراکسیداز و کاهش معنی‌داری در میزان فعالیت آنزیم کاتالاز مشاهده می‌شود. همچنین در تنش ویروس به همراه باکتری میزان فعالیت آنزیم‌ها در همه موارد با مقداری کاهش نسبت به گیاهان تحت تنش ویروس به تنهایی همراه بوده‌‌است.

کلیدواژه‌ها


عنوان مقاله [English]

The defence related enzymatic content of suger beet against Beet severe curly top virus and Pseudomonas fluorescens strain CAH0

نویسندگان [English]

  • Efat ALEMZADEH
  • Keramat IZADPANAH
  • Moussa ZAREI
  • Ali Akbar BEHJATNIA
چکیده [English]

Various environmental stresses including biotic and abiotic stresses lead to the production of reactive oxygen species (ROS). Accumulation of reactive oxygen species often have been detected in plant pathogen- interactions. Plants with a combination of enzymatic and non-enzymatic antioxidant systems usually ROS levels in cells kept in moderation. The aim of the present work is to study the effect of BSCTV infection and Pseudomonas fluorescens strain CAH0 on the activities of antioxidant enzymes in sugar beet plants by using a UV / Vis 2100 spectrophotometer. The results of the study showed when severe symptoms of the infection developed on the leaves and the virus content reached its maximum, a significant increase in the activities of several antioxidant enzymes, ascorbate peroxidase and peroxidase and a significant decrease in the developed on the leaves and the virus content reached its maximum, a significant increase in the activity of antioxidant enzyme, Catalse, was found. Also in virus's tension associated with bacteria, enzyme activity was reduced in all items.

کلیدواژه‌ها [English]

  • BSCTV
  • Antioxidant enzymes
  • Pseudomonas fluorescens strain CAH0

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