تأثیر ترکیبات فرار و غیر فرار جدایه‏هایTrichoderma spp. در جلوگیری از رشد پرگنه قارچ Fusarium solani عامل پوسیدگی خشک فوزاریومی سیب‏زمینی

نویسندگان

چکیده

با توجه به قابلیت بالای قارچ Trichodermaدر کنترل بیولوژیک بسیاری از عوامل بیماری زا و با توجه به اینکه قارچ Fusarium solani، عامل پوسیدگی خشک‏ سیب‏زمینی خسارات زیادی را به محصول وارد می سازد، در این تحقیق اثر هفت گونه از قارچ Trichoderma شامل چهار جدایهT. harzianum (1, 2, 3, 4) و جدایه‏های T. virens ,T. longibrachiatum ,T. koningiiعلیه بیمارگر در شرایط آزمایشگاهی مورد مطالعه قرار گرفت. آزمون بررسی اثر ترکیبات فرار جدایه‏های Trichoderma در جلوگیری از رشد پرگنه قارچ بیمارگر در حالت کشت Trichoderma ، 48 ساعت قبل از کشت F. solani به روش پتری های روی هم انجام گرفت و مشخص شد که ترکیبات فرار کلیه جدایه های مورد آزمایش، از رشد میسلیومی قارچ بیمارگر جلوگیری به عمل آوردند و جدایه  T. longibrachiatumبیشترین و جدایه‏یT. harzianum3 کمترین فعالیت آنتی بیوزی را در بازدارندگی از رشد میسلیوم قارچ F. solani داشته است. در بررسی تأثیر ترشحات مایع خارج سلولی جدایه‏های Trichoderma مشخص شد که ترشحات مایع جدایه‏هایT. harzianum (1, 2) به میزان پنج و ده میلی‏لیتر به ترتیب به میزان 76/9 و 28/14 درصد بیشترین تأثیر را در بازدارندگی از رشد میسلیومF. solani  داشته است.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of Volatile Metabolites and Non Volatile Metabolites of Trichoderma in preventing the growth of Fusarium solani the causal agent of Fusarium dry rot of potato

نویسندگان [English]

  • Zahra Taghizadeh
  • Sedighe Mohammadi
  • Hosein Alaie
چکیده [English]

Fusarium solani the causal agent ofFusarium dry rot of potato has a wide host range and worldwide distribution. Trichoderma is the most commonly used fungal biological control agent and have long been known as effective antagonists against plant pathogenic fungi. In present study volatile and non-volatile compounds produced from seven isolates of Trichoderma species including        T. harzianum (1, 2, 3 and 4), T. virens, T. koningii and T. longibrachiatum were avaluated for their antagonistic effects in vitro against F. solani. The volatile compounds produced form all antagonistic isolates showed mycelial inhibition of the test fungi. T. longibrachiatum and T. harzianum 3 revealed the highest and the lowest antibiosis activity against mycelial growth, respectively. Investigating the effect of non-volatile compounds at 5 and 10 ml concentration showed that the highest inhibitory percentage on mycelium growth of the pathogen caused by      T. harzianum 1 (14.28%) and T. harzianum 2 (9.76%) respectively.

کلیدواژه‌ها [English]

  • Antagonistic mechanisms
  • Trichoderma
  • Fusarium isolates

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