پژمردگی فوزاریومی سیبزمینی Fusarium oxysporum f.sp. tuberosi، یک بیماری مهم سیبزمینی است، که باعث زیان و کاهش قابل ملاحظهای در تولید میشود. منابع ژنتیکی مقاومت، بهترین روش کنترل بیماری است. در این تحقیق، عکس العمل 18 رقم سیبزمینی با استفاده از طرح فاکتوریل با 4 تکرار در شرایــط گلخانهای مورد ارزیابی قرار گرفت. زمانی که ارتـفاع گیاهچهها به 15 سانتی متر رسید با سه جدایه F. oxysporum ریشهها به میزان 100 میلی لیتر از سوسپانسیون (105 × 4 اسپور در هر میلی لیتر) در دو نوبت تلقیح گردیدند. ده روز تا یک ماه بعد از تلقیح ارقام، براساس شاخص علائم ظاهری بیماری، طول نکروز در آوندها، ارتفاع ساقه و طول ریشهها ارزیابی شدند.بین پارامترها، اختلاف معنیداری در سطح 1% وجود داشت. بیشترین مقدار همبستگی در روش ارزیابی مربوط به طول نکروز در آوندها (86/0= r)، طول ریشهها (75/0= r)، شاخص علائـــــم ظاهری (73/0= r) و ارتفاع ساقهها (69/0= r) بود. بین ارقام، اختلاف معنیداری وجود داشت (01/0= P). بــراساس طول نکروز در آوندها، ارقام در 5 گروه قرار گرفتند. ارقام گوریکان، تیوا، دیامونت، مارفونا، بارکا، آئولا و مورن در گروه بسیار حساس و ارقام اولوا، فولوا و آلوا درگروه متحمل قرار گرفتند.
Susceptibility assessment of potato cultivars to Fusarium wilts
نویسندگان [English]
Mehdi Nasresfahani؛ Rasoul Sharifi؛ Narges Nasrollahi؛ Shima Ramezani
چکیده [English]
Fusarium wilt (Fusariumoxysporumf.sp. tuberose) is a major disease of potato, causing considerable damage and losses. The sources of genetically resistant are the best method of controlling the disease. In this study, the reactions of 18 potato cultivars wereassessed to F. o f.sp. tuberosiin a factorial design with four replicates, in the greenhouse.Plants were inoculated (15 cm height) twice with three isolates of the pathogen by root inoculation (100 ml of 4×105 spore/ ml).Tento thirty days after inoculation cultivars were evaluated according to the symptoms disease index, the lengths of necrosis in vascular tissues, stem height and roots lengthsThere was a significant difference between parameters(P= 0.01). Necrosis lengths had maximum correlation in vascular tissues (r = 0.86), then root lengths(r = 0.75), symptom disease index scale (r = 0.73), and stem heights(r = 0.69). There was a significant difference between cultivars(P= 0.01). According to lengths of necrosis in vascular tissues cultivars divided in five groups, Gorican, Tiuva, Diamont, Marfona, Baraka,Aola and Moren were very susceptible and Olva, Folva and Alva were tolerant.
کلیدواژه‌ها [English]
Potato, Cultivars susceptibility, Fusarium wilt, Potato plant Fusariumoxysporumf.sp. tuberose
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