بررسی دزهای کشنده جدایه ایرانی قارچ Beauveria bassiana در کنترل میکروبی بید آرد (Lep.: Pyralidae) Ephestia kuehniella روی

نویسندگان

چکیده

بید آرد Ephestia kuehniella از آفات مهم انباری می­باشد. این تحقیق به ­منظور بررسی کارایی جدایه ایرانی مناسب قارچ Beauveria bassiana در کنترل میکروبی شب­پره آردEphestia kuehniella روی خرمای رقم سایر انجام شد. برای تعیین قدرت کشندگی قارچ روی مراحل تخم، لارو و شفیره، پنج دُز 104،104×5، 105، 105×5 و 106اسپور در میلی­لیتر  به­روش غوطه­ورسازی استفاده شد. داده­ها با استفاده از روش لگاریتم-پروبیت برازش­گردیدند. نتایج نشان داد که بیش­ترین دُز کشنده 50 درصد مربوط به جدایه   IRAN441Cروی شفیره 104 × 76/1 و کمترین دُز کشنده 50 درصد مربوط به مرحله تخم و معادل103×49/3 اسپور در میلی­لیتر بود. بالاترین و پایین‌ترین زمان50 درصد کشندگی به­ترتیب برای شفیره و تخم و معادل  67/3 و 38/3 روز بود. به­طورکلی نتایج تحقیقات در فاز آزمایشگاهی که قدم نخست در امکان‌سنجی کاربرد این قارچ در کنترل میکروبی بید آرد می­باشد، نشان داد که قارچ عامل بیمارگر از پتانسیل کاربرد بالایی برخوردار است.

کلیدواژه‌ها


عنوان مقاله [English]

Study the lethal doses of suitable isolate of Beauveria bassiana for microbial control of Ephestia kuehniella on Sayer Date cultivar

نویسندگان [English]

  • Negar Bahmani
  • Hadi Ostovan
  • Masoud Latifian
  • Bahar Rad
چکیده [English]

The Ephestia kuehniella is one of the important pest of storage Date. This research was conducted to evaluating the efficiency the suitable Iranian isolate of Fungus Beauveria bassiana for the microbial control the flour moth on the Sayer date cultivar. The larvae were treated by 5 doses of fungi including 104,5×104,105, 5×105 and 106spores/ml to determine the lethal power, with dropping method. Data were calculated by using Log-Probit analysis methods. Results showed  that the highest and lowest LC50 were for pupa and egg equivalent to 1.76×104 and 3.49×103 spores/ml respectively. The lowest and highest LT50were assessed for pupa and egg equivalent to 3.67 and 3.38 days respectively. The research results of in the laboratory phase as the first step of feasibility of fungus application in microbial control showed that fungal pathogen had high potential for application on control of flour moth in date

کلیدواژه‌ها [English]

  • Microbial control
  • flour moth
  • date palm

Akbar W., Lord J.C., Nechols J.R., Howard R.W. 2004. Diato­maceous earth increases the efficacy of Beauveriabassianaagainst Triboliumcastaneumlarvae and increases conidia at­tachment. Journal of Economic Entomology. 97 (2): 273–280.

Bagheri-Zenouz, E. 2007.Pests of Stored Products and Management to Maintain Bioecology of Insects, Acari and Microorganism. University of Tehran Publication, Iran

Batta Y.A. 2004. Control of the rice weevil (SitophilusoryzaeL., Coleoptera: Curculionidae) with various formulations of Metarhiziumanisopliae. Crop Protection. 23 (2): 103–108.

Beegle, C. C. & YamamotoT. 1992. Invitation paper C.P. Alexander fund. History ofBacillus thuringiensisBertiner research and development Canadian Entomologist 124 587-616.

Boucias, D. G. &PendlandJ. C. 1998. Principles of Insect Pathology. Kluwer Academic Publishers. Boston, Masachusetts. 491Pp.

Butt, T. M. & M. S. Goettel. 2000. Bioassay of entomogenous fungi. Pp. 141-195In: Navon, A. and K. R. S. Ascher(eds.).Bioassay ofEntomopathogenicMicrobes and Nematodes. CABI publishing, U. K.                           

Charnley, A. K.1992. Mechanisms of fungal pathogenesis in insects with particular reference to locusts. In C. J. Lomer and C. Prior: (Eds)Biological control of locusts and grasshoppers, proceeding of a workshop held at International Institute of Tropical AgricultureCotonou, Republic ofBenin. CAB International UK.

Daoust, R. A. & RobertsD. W.1983. Studies on the prolonged storage of Metarhiziumanisopliaeconidia effect of growth substrate on conidial survival and virulence against mosquitoes. J. Inverteb. Path. 41: 161-170.

Dehoog, G. S.1972. The genera Beauveria, Isaria, Tritirachiumand Acrodontium gen. Nov. Studies in Mycology (CBS) 1:1-41

Fragues, J., DelmasJ. C.& LebrunR. A. 1994. Leaf consumption by larvae of Colorado potatoes beetle(Coleoptera: Chrysomelidae) infected with the entomopathogen, Beauveriabassiana. Pp. 23-69In: Butt, T. M., Jackson, C. and Magan, N. (Eds.) Fungi as biocontrol agents, Progress, Problems and Potentials. CAB International, Wallingford UK. 

Ghazavii, M. Kharazi-Pakdel,A.Ershad,J.&Bagherizenouz,E. 2002. Effeciency of Iranian isolates of BeauveriabassianaagainsetLocustamigratoria (Orthoptera: Acrididae). Applied Entomology and Phytopathology. 69(2): 111-128

Gillespie, A. T.& Clayton,N.1989. The use of entomogenous fungi for pest control and the role of toxin in pathogenesis. Pesticides Science. 27: 203-215.

Gomez K. A.&Gomez A. A. 1984.Statistical Procedures for Agricultural Research. 2nd end. New York, NY, USA: John Wiley and Sons. Pp. 116–140.

Gross, H. R. JR., Pair, S.D.& JacksonR.D. 1985. Behavioral responses of primary entomophagous predators to larval homogenates of Heliotbiszea and Spodopterafrugiperda (Lepidoptera: Noctuidae) in whorl-stage corn. Journal of  Environmental Entomology, 14:360-364

Hassan, L. S., &Steenberg,T.2007. Combinating larval parasitoids and entomophatogenic fungus for biological controlofSitophilus granaries in stored grain.Biological Control, 40: 237-242.

Horak M. 1994. A review of cadrawalker in Australia: five new native species and the two introduced pest species (Lepi­doptera: Pyralidae: Phycitinae). Australian Journal of Entomology, 33 (3): 245–262.

Iwana, R. &AshidaM. 1986. Biosynthesis of prophenoloxidase in hemocytes of larval hemolymph of the silkworm, Bombyxmori. Insect Biochemistry, 16:547-555.

James, E. T. & Lord, J. C.2003. Tritrophic interactions and storage pest control: interaction of the fungus Beauveriabassiana with resistant oat varieties for control of Oryzaephilussurinamensis. Insect Pathology and Microbial Control, 4: 153-170

Jassim, H. K., Abdullah,L. M.&Abd-Al-Ahad, I. 1998. Determination of the exact concentration of Beauveriabassiana to control the larvae of the Fig moth Ephestiacautellaon stored dates in Iraq. Arab Journal of Plant Protection, 6: 44-45

Kawakami, K. 1987. The use of an entomogenous fungus Beauveriabrongniartii, to control the yellow- spottedlongicorn beetle, psacotbeabilaris. Extension Bulletin, ASPAC Food and fertilizer technology center for the Asian and pacific Region (1987) No. 257, Pp. 38-39.

Kaya, G. P. 1993. Humeral Antibacterial Immunity in Insect Immunity. Kluwer Academic Press, London. Pp 93-115.

Latifian, M. 2004. Date palm Stored Pests,Control Technology. AhangGhalamPublicher. Mashhad,Iran

Latifian, M.,Soleimannejadian,E.Ghazavi, M.,Hayati, J.,Mosadegh,S, M. &Nikbakht,P.2009. Evaluation of three Beauveriabassiana isolates on Sawtoothed beetle Oryzaephilussurinaemensis and the effect of different temperature on their germination and mycelium growth. 77(1): 151-168.

Mirshekar, A. M. Ghazavi. P. Azmayeshfard. & A. Kharazi-Pakdel. 2004. Comparative and laboratory study of pathogencity of Beauveriabassiana and Metarhiziumanisopliae on Chrotogonustrachypterus. 68(1): 141-156.

Moore D., Lord J.C.& Smith S.M. 2000. Pathogens. p. 193–227. In: “Alternatives to Pesticides in Stored-Product IPM” (Bh. Subramanyam, D.W. Hagstrum, eds.). Kluwer Academic Publishers, Dordreecht, 193 Pp.

Navon, A. & K. R. S. Ascher. 2000. Bioassay of EntomophathogenicMicrobes and Nematodes.CABI publishing. 324Pp.

Padin, S. B., Dal belloG. M. &Vasicek, L.1994. Bioinsecticide Potential of EntomopathogenicFungi in Stored Grain Pests. Rev. Fac. Agron. Univ. Nac. La Plata. 15: 1-7.

Pezhman, H. 2001. Guidness of Cultivation, Sanitation and Harvesting of Date palm.AhangGhalamPublicher. Mashhad,Iran

Sabbour M.M., Abd-El-Aziz Sh.E. 2010. Efficacy of some bioin­secticides against Bruchidius incarnates (Boh.) (Coleoptera: Bruchidae) infestation during storage. Journal of Plant ProtectionResearch, 50 (1): 25–31.

Steinhaus, E. A. 1967. Insect Microbiology. New York and London: Hafner Publishing Co.

Webb, S.E. & Shelton,A.M. 1990. Effect of age structure on the outcome of viral epizootics in field populations ofimported cabbage worm (Lepidoptera: Pieridae). Environmental Entomology, 19:111-116.

Yoshinori, T. & Kaya,H. K. 1992. Insect Pathology. Academic Press. USA