عنوان مقاله [English]
نویسندگان [English]چکیده [English]
In 2010 and 2011 surveys of Fars provience were observed symptoms doubtful to phytoplasma diseases such as virescence and phyllody, floral proliferation, witches’-broom, yellowing and little leaf in eggplant fields. To determination of etiology this symptoms, seven infected eggplant plants from Fasa, Jahrom, Firouzabad, Darab and Sarvestan were planted in suitable pots, transferred to a greenhouse without any insects and used as sources of disease for further studies. The causal agents were transmitted from diseased to healthy eggplants by grafting and from eggplant to periwinkle by dodder. In inoculated eggplants, were appeared big bud, little leaf, yellowing and witches’-broom and in inoculated periwinkles, were appeared virescence, yellowing and witches’-broom. Total DNA were extracted from fresh midrib tissues of naturally infected eggplant, experimentally inoculated periwinkle plants and a healthy eggplant and periwinkle plant. In direct PCR using primer pairs P1/P7, DNA fragments of approximately 1800 bp were amplified from total nucleic acid samples extracted from symptomatic eggplant and periwinkle plants. Periwinkle reaction to inoculation with Eggplant Big Bud (EBB) different isolates was variable as based on difference in severity and type of symptoms in periwinkle, EBB different isolates, were placed in six groups. Direct PCR products of EBB different isolates were digested with AluI, HaeIII and RsaI enzymes. Comparison of RFLP patterns indicated that EBB different isolates place in two groups: Fasa 1, Fasa 2, Fasa 3, Firouzabad, Darab and Sarvestan isolates in one group and Jahrom isolate in other group. Based on disease symptoms, dodder and graft transmission and positive reaction in PCR, eggplant big bud in Fars province has phytoplasmal etiology. This is the first report of the presence of EBB in Fasa, Firouzabad, Darab and Sarvestan. With molecular testings in this study, were confirmed there are at least two phytoplasmas associated with EBB in Fars province. By using more restriction enzymes, it is likely to see more diversity in EBB agent in Fars province.
Amaral Mello, AP. Bedendo, IP. Kitajima, EW. Ribeiro, LFC. & Kobori, R. 2006. Tomato big bud associated with a phytoplasma belonging to group 16SrIII in Brazil. International Journal of Pest Management, 52: 233-237.
Anonymous, 2011. Agriculture Statistics First Vol., Crops 2009-10. Persian Gerafic Press, Tehran, Iran.
Dehghani, A. & Salehi, M. 2011. Short report of tomato big bud disease in Lorestan province. Iranian Journal of Plant Pathology, 483: 47.
Jamshidi, E. 2011. Detection and Characterization of Phytoplasmas Associated with Big Bud Disease of Tomato Using Molecular Methods in Khorasan Razavi and Other Provinces. M.Sc. Thesis, Department of Agriculture, Ferdowsi University of Mashhad, Khorasan Razavi, Iran.
Lee, IM. Gundersen-Rindal, DE. Davis, RE. & Bartoszyki, IM.1998. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. International Journal of Systematic Bacteriology, 48:1153-1169.
Omar, AF. & Foissac, X. 2012. Occurrence and incidence of phytoplasmas of the 16SrII-D subgroup on solanaceous and cucurbit crops in Egypt. Biomedical and Life Sciences European Journal of Plant Pathology, 133: 353-360.
Peyvast, G. 2009. Olericulture. Daneshpazir Press, Gilan, Iran.
Raoofi, D. 2010. Biological and Molecular Characterization of Tomato and Eggplant Phytoplasma Disease Agent (Agents) in Bushehr Province. M.Sc. Thesis, Islamic Azad University, Semnan Branch, Semnan, Iran.
Raoofi, D. Salehi, M. & Firouz, R. 2010. Multiple phytoplasma associated with eggplant witches broom in Bushehr province. Proceedings of the 19th Iranian Congress of Plant Protection, 12-9 Aug. 2010, Tehran, Iranian Plant production Research Institute, p. 427.
Rashidi, M. 2006. Occurrence tomato big bud disease in Isfahan, Ardabil and West Azarbaijan provinces . Proceedings of the 17th International Congress of Plant Protection, 11-14 Sept. 2006, College of Agriculture and Natural Resources, Tehran University, p. 188.
Salehi, M. & Izadpanah, K. 1995. Big Bud disease in tomato and eggplant in Fars. Proceedings of 12th Iranian Congress of Plant Protection, 16-11 Sept. 1995, Karaj, College of Agriculture, p. 169.
Salehi, M. Babani, G. & Izadpanah k. 2000. Eggplant Witches Broom in Bushehr and Fars provinces. Proceedings of the 14th Iranian Congress of Plant Protection, 14-17 Sept. 2000, Isfahan University of Technology, p. 318.
Salehi, M. Izadpanah, k. & Heydarnezhad, J. 2006. Identification and grouping of 35 phytoplasmas Based on molecular properties from central and southern areas of Iran. Iranian Journal of Plant Pathology, 423: 51-52.
Schneider, B. Cousins, MT. Klinkong, S. & Seemüller, E. 1995. Taxonomic Relatedness and Phylogenetic Positions of Phytoplasmas Associated With Diseases of Faba Bean, Sunnhemp, Sesame, Soybean, and Eggplant. Journal of Plant Diseases and Protection, 102: 225-232.
The IRPCM Phytoplasma/Spiroplasma Working Team - Phytoplasma taxonomy group. 2004. Candidatus Phytoplasma, a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects. International Journal of Systematic and Evolutionary Microbiology, 54: 1243-1255.
Torabi, E. 1994. Etiological Review of Big Bud Disease in Azerbaijan. M.Sc. Thesis, Department of Agriculture, Tarbiat Modarres University, Tehran, Iran.
Zhang, Y.P. Uyemoto, J.K. & Kirkpatrick, B.C. 1998. A small scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay. Journal of Virological Methods, 71: 45-50.