ارزیابی توانایی کشندگی قارچ Metarhizium anisopliae در مقابل موریانه Microcerotermes diversus (Isoptera : Termitidae)

نویسندگان

چکیده

مخرب‏ترین موریانه در استان خوزستان گونه Microcerotermes diversus (Isoptera : Termitidae)  می‏باشد. در این پژوهش توانایی کشندگی قارچ Metarhizium anisopliae، جدایه سراوان (DEMI 001) در مقابل موریانه M. diversus در آزمون زیست‏سنجی غیرانتخابی به دو روش آغشته‏سازی کاغذ صافی و غوطه‏ورسازی موریانه‏ها در سوسپانسیون اسپور قارچ و همچنین در آزمون زیست‏سنجی انتخابی مورد بررسی قرار گرفت.  نتایج آزمایش‏ها نشان داد که غلظت و زمان کشندگی در روش آغشته‏سازی مقادیر بالاتری را در مقایسه با روش غوطه‏ورسازی دارند. کمترین میزان LC50 و LT50 مربوط به آزمون زیست‏سنجی غیرانتخابی به روش غوطه‏ورسازی بود که به ترتیب برابر با 104×1/3 اسپور در میلی‏لیتر و 02/1 روز بودند. میزان تغذیه از کاغذ صافی تیمار شده و تیمار نشده در آزمون انتخابی به خوبی نشان داد که سوسپانسیون اسپور قارچ مورد نظر در غلظت‏های مورد استفاده در این تحقیق برای موریانه‏ هدف دورکنندگی نداشت. در مجموع این پژوهش نشان داد که میزان کشندگی قارچ بیمارگر M. anisopliaeدر مقابل موریانه M. diversusبا روش ارائه قارچ در جمعیت ارتباط دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of fatality potential of fungus Metarhizium anisopliae against the termite Microcerotermes diversus (Isoptera : Termitidae)

نویسندگان [English]

  • Amir Cheraghi
  • Behzad Habibpour
چکیده [English]

Microcerotermes diversus (Iso.: Termitidae) is one of the most destructive termites in Khuzestan province (Iran). In this study, ability of pathogenicity of the fungus Metarhizium anisopliae strain of Saravan (DEMI 001) against termite M. diversus was investigated in choice and no- choice bioassay trials by smeary and immersion methods. Results showed that concentration and time of fatality in the smeary method was higher than the immersion method. The lowest level of LC50 and LT50, it related to bioassay test no- choice with immersion method. Those level were 3.1 × 104 spore Ml-1 and 1.02 day, respectively. The feeding of treated and untreated filter paper in choice test showed that fungal conidial suspension in concentrations used in this study had no repellency effect against the target termite. Overall, this study showed that the fatality rate of fungal pathogen. M. anisopliae against termite M. diversus was associated with the fungus population.
 

کلیدواژه‌ها [English]

  • bioassay
  • Entomopathogen fungus
  • Biological control
  • Termite

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