شناسائی و پراکنش عوامل بیماری‌زای ویروسی مولد موزائیک در مزارع لوبیاچیتی استان اصفهان

نویسندگان

چکیده

در یک بررسی سه‌ ساله نمونه‌هایی با نشانه‌های  ابلقی، موزائیک، زردی و بدشکلی برگ‌ها از مزارع لوبیا چیتی در مناطق مختلف استان اصفهان جمع­آوری گردید. نشانه‌های حاصل از مایه زنی شیره نمونه‌ها روی گیاهان آزمون و نتایج بدست آمده از انجام آزمون سرولوژیک الیزا، نشانگر آلودگی نمونه‌های مذکور به ویروس موزائیک معمولی لوبیا (Bean Common mosaic virus, BCMV)، ویروس موزائیک خیار (Cucumber mosaic virus, CMV)، ویروس موزائیک یونجه (Alfalfa mosaic virus, AMV) و ویروس پیچیدگی برگ چغندر (Beet curly top virus, BCTV) بود. فراوانی ویروس‌های مزبور با نمونه برداری از مرحله 4-2 برگی تا مرحله گلدهی تعیین گردید و مشاهده شد که ویروس موزائیک معمولی لوبیا در تمام مناطق لوبیا کاری استان از شایع‌ترین عوامل ویروسی است. آلودگی اولیه بوته‌ها در مرحله 4-2 برگی به این ویروس  بین 41/0 تا 31/2 درصد و در مرحله گلدهی بین 17/3 تا 16/23 درصد تعیین شد. فراوانی آلودگی مزارع لوبیا به ویروس‌های موزائیک خیار و موزائیک یونجه در مرحله 4-2 برگی به ترتیب بین صفر و 17/0 درصد و در مرحله گلدهی به ترتیب بین 74/2 و 92/8 درصد بود. همچنین 34/1 درصد ازگیاهچه های پرورش یافته از  بذرهای جمع آوری شده از مزارع مختلف لوبیا چیتی آلوده به ویروس موزائیک لوبیا و 02/0 درصد آلوده به ویروس موزائیک خیار بودند. آلودگی به ویروس موزائیک یونجه در این گیاهچه ها مشاهده نگردید. 

کلیدواژه‌ها


عنوان مقاله [English]

Identification and distribution of viruses in the French bean fields of Isfahan province

نویسندگان [English]

  • Sadegh Jalali
  • Kaveh Bananej
چکیده [English]

In a 3-year investigation on french bean fields, samples with mosaic, mottling and yellowing symptoms were collected from French bean fields in various regions of Isfahan province .The symptoms resulted from inoculating samples to test plants and serological tests, indicated that the samples infected to Bean common mosaic virus (BCMV) , Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV) and Beet curly top virus (BCTV). Frequency of the viruses was determined through sample collecting from  2 - 8 leaf till flowering stages it was indicated that 121BCMV was the prevalent virus in all French bean fields. Infection rate to BCMV in 2-4 leaf stage was 0.41-2.31% and in flowering stage was 3.7-23.16%.Infection of bean fields to CMV and AMV in 2-4 leaf stage were 0 and 0.17% and in flowering stage were 2.74 and 8.92%, respectively.In seed transmission test, 1.34% and 0.02% were infected to BCMV CMV respectively, and infection to AMV was not observed in the seedlings emerged from fied collected bean seeds.

کلیدواژه‌ها [English]

  • Frenchbean
  • virus
  • identification
  • Dispersion
  • Isfahan
Acikgoz, Z. & Citir, A. 1986. Incidence, epidemiology and viruses on Phaseolus vulgaris. Journal of Turkish Phytopathology, 15:61-76 .

Al-Fadhil, F. H. & Al-Ani, R. A. 1988. Effect of plant age on developement of common bean mosaic and yield. Journal of Agricalture and Water Resource, 7:75-84.

AL-Musa, A. M. 1989. Oversummering hosts for some cucurbit viruses in the Jordan Valley. Phytopathology, 127;49-54.

Bennett, C.W. 1979. The Curly Top Disease of Sugarbeet and Other Plants. Monograph No.7, American Phytopathological Society, USA.

Bos, L. 1971. Bean Common Mosaic Virus. Description of Plant Viruses,  C.M.I. /A.A.B. , No73.

Davis, R. F. & Hampton, R. O. 1986. Cucumber mosaic virus isolates seedborn in Phaseolus vulgaris: serology, host-pathogen relationships and seed transmission. Phytopathology,76: 999-1004.

Dizadji, A., Shahraeen, N. & Sahandi, A. 2008. Detection of viruses infecting bean in East Azarbaijan fields. 18thIranian Plant Protection Congress, University of Bu-Ali Sina, Volume II, Plant Diseases.

Drijfhout, E. 1991. Bean common mosaic virus. p. 37-39,  In: Compendium of Bean Diseases, Hall, R., Led. APS Press, USA.

Hall, R.1991. Compendium of Bean Diseases. APS Press, USA.

Halbert, S. E., Mink, G. I., Silbernagel M. J. & Mowry, T. M. 1994. Transmission of bean common mosaic virus by cereal aphids (Homoptera ). Plant Disease 78:983-985.

Hormozi-nejad, M. H., Mozafari J. & Rakhshandehroo F. 2008. Responses of Iranian bean cultivars to Bean common mosaic virus(BCMV). 18thIranian Plant Protection Congress, Univ. of Bu-Ali Sina, Volume II, Plant Diseases.    p 519.

Hoyle, B. J. 1977. Curly Top Identification Hand Book in Nine Crops. University of California, Publication No.4079, USA.

Izadpanah, K. 1981. Complete List of Plants Viral and Viral like Diseases in Fars. Shiraz University Press. 171pp.

Jalali, S. 2001. Ivestigation on dissemination of Beet curly top virus and identification of its other cultivated host in Isfahan province. Journal of Sugar Beet, 17: 121-132.

Jilaveanu, A. 1988. Experimental data on the economic importance, spread and variability of bean common mosaic in Romania. Problem De Protectia Plant, 16:133-145.

Kaiser, W. J., Mossahebi G. M. & Okhvat, M. 1971. Alternative hosts of viruses affecting food legumes in Iran. Iranian Journal of Plant Pathology,7: 25-29.

Kaiser, W. J. & Hannan, R. M. 1983. Additional hosts of alfalfa mosaic virus and its seed transmission in bean. Plant Disease, 67:1354-1357.

Klein, R. E., Wyath S. D. & Kaiser, W. J. 1989. Influence of propagation on incidence of seed borne bean common mosaic virus in USDA phaseolus germplasm collection. Plant Disease, 73: 759-761.

Peyambari, M., Koohi Habibi, M., Mosahebi, Gh. & Izadpanah K. 2006. Serological detection of bean common mosaic and bean common mosaic necrosis viruses in several provinces, report of white bean as a tolerant genotype and rate of BCMNV seed transmission in three bean genotypes. 17thIranian Plant Protection Congress, University of Tehran, Karaj. Volume II, Plant Diseases, p. 132.

Purcifull, D. E. & Batchelor, D. H. 1977. Immunodiffusion tests with  sodium dodecyl sulfate (SDS)- treated plant viruses and plant viral inclusions. Florida Agricultural Experiment Station Technical Bulletin, No. 788.

Provvidenti, R.1976. Reaction of Phaseolus and Macroptillium species to a strain of Cucumber mosaic virus. Plant Disease Reporter, 60:289-293.

Shahraeen, N. 1993. High incidence of virus diseases in phaseolus bean in Zanjan province. 11thIranian Plant Protection Congress,University of Guilan, Rasht, Iran, p. 142.

Silbernagel, M. J.1965. Differential tolerance to curly top in some snap bean varieties. Plant Disease Reporter, 49:475-477.

Thomas, H. R.1951.Yellow dot, a virus disease of bean. Phytopathology,41:967-974.

Vishwa, D. & Gurha, S. N. 1990. Effect of BCMV on yield and yield attributes  in french bean. Journal of Pules Reseach, 3:89-91.

Zaumeyer, W. J. 1963. Two new strains of alfalfa mosaic virus systemically infection to beans. Phytopathology, 43:38-42.